Document Type: Original Article
Radiopharmaceutical Research and Development Laboratory, Nuclear Science and
Technology Research Institute, Atomic Energy organization of Iran, Tehran, Iran
Agricultural, Medical and Industrial research School, Nuclear Science and Technology
Research Institute, Atomic Energy organization of Iran, Karaj, Iran
Introduction: [61Cu]diacetyl-bis(N4-methylthiosemicarbazone) ([61Cu]ATSM) is a well-established hypoxia imaging tracer with simple production and significant specifity. In this work the accumulation of the tracer is studied in wild-type, necrotic and hypoxic fibrosarcoma tumors.
Methods: [61Cu]ATSM was prepared using ATSM ligand and [61Cu]CuOAc followed by i.v. administration and imaging studies in wild-type rats and hypoxic fibrosarcoma-bearing mice.
Results: [61Cu]ATSM with high radiochemical purity (>99%, HPLC, RTLC) was injected to wild-type rats as well as hypoxic and necrotic fibrosarcoma-bearing mice followed by imaging up to 3 hours.
Conclusion: [61Cu]ATSM was mainly accumulated in liver, as well as kidney and bladder and less but still significant in brain of wild-type rats. A significant and hypoxia-specific tumor/non tumor ratio in hypoxic models was observed by co-incidence imaging 2 h post injection, while in necrotic and 12-week tumor-induced mice very slight tumor uptakes were detected. [61Cu]ATSM is a positron emission tomography (PET) radiotracer for selective tumor hypoxia imaging from necrotic and proliferative tumors.