Production, quality control and imaging of 64Cu-ATSM in healthy rabbits for clinical applications

Fazaeli, Yousef; Jalilian, Amir Reza; Kamali-Dehghan, Mohsen; Bolourinovin, Fatemeh; Moradkhani, Sedigheh; Aslani, Gholamreza; Rahimnejad, Ali; Ghannadi-Maragheh, Mohammad

Production, quality control and imaging of 64Cu-ATSM in healthy rabbits for clinical applications

Document Type : Original Article

Authors

Yousef Fazaeli ¹

Amir Reza Jalilian ²

Mohsen Kamali-Dehghan ¹

Fatemeh Bolourinovin ¹

Sedigheh Moradkhani ¹

Gholamreza Aslani ¹

Ali Rahimnejad ¹

Mohammad Ghannadi-Maragheh ²

¹ Nuclear Medicine Research Group, Agricultural, Medical and Industrial Research School, Nuclear Science and Technology Research Institute, Atomic Energy organization of Iran,Karaj, Iran

² Radiopharmaceutical Research and Development Laboratory, Nuclear Science and Technology Research Institute, Atomic Energy organization of Iran, Tehran, Iran

Abstract

Introduction: [⁶⁴Cu]diacetyl-bis(N⁴-methylthiosemicarbazone) ([⁶⁴Cu]ATSM) is a well-established hypoxia imaging tracer with reproducible production and significant specifity. In this work the high yield production and quality control as well as imaging studies in healthy rabbits is reported.
Methods: Copper-64 produced via the ⁶⁸Zn(p,an)⁶⁴Cu nuclear reaction (30 MeV protons at 180 µA) was used for the preparation of [⁶⁴Cu]diacetyl-bis(N4-methylthiosemicarbazone)([⁶⁴Cu]ATSM). Followed by quality control and administration to healthy rats as well as healthy rabbits for biodistribution and imaging studies respectively.
Results: ⁶⁴Cu²⁺ (500 mCi, separation yield> 95%, radionuclide purity>96%) was used for [⁶⁴Cu]ATSM production (radiochemical purity>99%, specific activity of 300 Ci/mmol) followed by administration to healthy rabbits and coincidence imaging demonstrating uptake in liver, kidney and bowel as shown by other reports in various rodents and human.
Conclusion: [⁶⁴Cu]ATSM radiopharmaceutical is produced and now available in large quantities for research and/or clinical trials in the country.

Keywords

Radiolabeling

quality control

[64Cu]ATSM

Rabbits

Biodistribution

Coincidence imaging

Subjects