Department of Radioisotope, Nuclear Research Center, AOEI, Tehran, Iran
Abstract
Imaging of infection and inflammation is an important issue in nuclear medicine as it may have relevant implication for the management of patients with infections or inflammatory diseases. In this regard the synthesis of human polyclonal immunoglobuline G (IgG) radiolabeled with technetium-99m (99mTc) by a novel method, via a nicotinamide hydrazine derivative, was performed. The biological behavior, stability and its high specific activity make this radiopharmaceutical a suitable reagent for radiolabeling of proteins and peptides. In this article the method for synthesis of a sterile and apyrogen 99mTc-HYNIC-hIgG kit is presented. The preparation of the kit consists two steps, in the first step 740 MBq pertechnetate was added in Kit No.1, which contains SnCl2 and tricine. After 5 minutes the aliquot in Kit No.1 was added to Kit No.2, which contains HYNIC-hIgG. 99mTc-HYNIC-hIgG complex is stable in cysteine and serum with a labeling efficiency more than 90% after one hour.
Babaei, M. H., Shafiei, M., Behradkia, P., & Najafi, R. (2004). Synthesis, formulation and quality control of 99mTc-HYNIC-hlgG for imaging focal sites of infection and inflammation in human subjects [Persian]. Iranian Journal of Nuclear Medicine, 12(1), 33-39.
MLA
Mohammad hossein Babaei; Mohammad Shafiei; Payam Behradkia; Reza Najafi. "Synthesis, formulation and quality control of 99mTc-HYNIC-hlgG for imaging focal sites of infection and inflammation in human subjects [Persian]". Iranian Journal of Nuclear Medicine, 12, 1, 2004, 33-39.
HARVARD
Babaei, M. H., Shafiei, M., Behradkia, P., Najafi, R. (2004). 'Synthesis, formulation and quality control of 99mTc-HYNIC-hlgG for imaging focal sites of infection and inflammation in human subjects [Persian]', Iranian Journal of Nuclear Medicine, 12(1), pp. 33-39.
VANCOUVER
Babaei, M. H., Shafiei, M., Behradkia, P., Najafi, R. Synthesis, formulation and quality control of 99mTc-HYNIC-hlgG for imaging focal sites of infection and inflammation in human subjects [Persian]. Iranian Journal of Nuclear Medicine, 2004; 12(1): 33-39.
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