Development of Sm-153 chitosan for radiosynovectomy

Bahrami-Samani, Ali; Jalilian, Amir Reza; Yousefnia, Hassan; Akhlaghi, Mehdi; Mazidi, Mohammad; Ghannadi-Maragheh, Mohammad

Development of Sm-153 chitosan for radiosynovectomy

Document Type : Original Article

Authors

¹ Radiopharmaceutical Research and Development Laboratory, Nuclear Science and Technology Research Institute, Atomic Energy organization of Iran, Tehran, Iran

² Research Institute for Nuclear Medicine, Tehran University of Medical Sciences, Tehran, Iran

Abstract

Introduction: Developing new radiosynovectomy agents is of great importance due to the aging of human populations around the world and increasing the incidence of inflammatory diseases. In this work, Sm-153 chitosan agent was developed for the first time in our country and preparation and quality control of the compound is described.
Methods: Sm-153 chloride was obtained by thermal neutron flux (4-5 × 10¹³ n.cm^-2.s^-1) of natural Sm2O3 sample, dissolved in acidic media. ¹⁵³Sm-samarium chloride (370 MBq) was used in preparation of ¹⁵³Sm-chitosan complex followed by quality control using MeOH: H2O: acetic acid (4: 4: 2) as mobile phase. The complex stability and viscosity were checked in the final solution up to 2 days. The complex solution and ¹⁵³Sm³⁺ (80 µCi/100 µl) were injected intra-articularly into male rat knee joint followed by scarification studies 6 d post injection.
Results: Sm-153 chitosan was prepared successfully with high radiochemical purity (>99%, ITLC) at room temperature after 10-30 min followed by autoclave sterilization. The complex was stable at room temperature and 37ºC up to 2 days. No significant leakage of dose from injection site and its distribution in organs were observed up to 6 days for ¹⁵³Sm-chitosan.
Conclusion: Approximately, more than 90% of injected dose remained in injection site after 6d. The complex is a dedicated agent for radiosynovectomy. The experience from this study would lead to the development of more sophisticated radiosynovectomy radiopharmaceutcals for human use in the country.

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