Production and quality control of 166Ho-Chitosan for therapeutic applications

Zolghadri, Samaneh; Jalilian, Amir Reza; Yousefnia, Hassan; Bahrami-Samani, Ali; Shirvani-Arani, Simindokht; Mazidi, Mohammad; Akhlaghi, Mehdi; Ghannadi-Maragheh, Mohammad

Production and quality control of 166Ho-Chitosan for therapeutic applications

Document Type : Original Article

Authors

¹ Radiopharmaceutical Research and Development Laboratory, Nuclear Science and Technology Research Institute, Atomic Energy organization of Iran, Tehran, Iran

² Research Institute for Nuclear Medicine, Tehran University of Medical Sciences, Tehran, Iran

Abstract

Introduction: In this research, [¹⁶⁶Ho]Holmium chitosan complex production is described in details, followed by determination of complex radiochemical purity, stability and biodistribution (after intra-articular injection) in wild-type male rats. Finally a Ho-166 based chitosan kit for ultimate radiosynovectomy as well as radiotherapy applications was developed. Methods: ¹⁶⁶Ho-chitosan complex was prepared using chitosan concentrations and ¹⁶⁶HoCl3 followed by intra-articular injection and biodistribution studies in wild-type rats including and excluding injected knee. Results: The [¹⁶⁶Ho]Holmium chitosan complex was prepared with high radiochemical yield (>95 %) in the optimized condition (35mg/3ml of chitosan in %1 AcOH, pH. 3, >98%, ITLC) was injected to wild-type rats followed by the biodistribution studies of the compound among the tissues excluding the injected knee data. Intra-articular injection of [¹⁶⁶Ho]holmium chitosan complex to male wild-type rats and investigation of leakage of activity in the body showed that most of injected dose has remained in injection site 144 h after injection. Conclusion: Successful development and formulation of ¹⁶⁶Ho-chitosan kit is described. This kit has the potential for use in clinical setting namely for radiosynovectomy and cancer radiochemotherapy.

Keywords