During the 6-m period, 24 patients with acute scrotal pain were referred to the Nuclear Medicine Department of Shaheed Labbafi-Nejad hospital. Their age ranged from 9 to 84 (mean 28.8) years. Twelve scans were selected for the study. The scans were performed on angiographic and static phases, using Tc-pertechnetate. Of the above mentioned cases, one had acute testicular torsion (pre and post operative scans), two had epididymitis, six had orchitis, and two had epididymo-orchitis. The results were verified by clinical follow up and surgery. This study indicates that isotopic scan is very sensitive and accurate in evaluation of acute scrotal pain and can be very helpful in emergency cases.