Radiopharmaceutical Research and Development Lab, Nuclear Science and Technology Research Institute, Atomic Energy Organization of Iran, Tehran, Iran
Abstract
Introduction: In order to develop a radiolabeled calcitonin (CT) derivative for receptor imaging studies, CT was successively labeled with 67Ga-gallium chloride. Methods: The best results of the conjugation were obtained by the addition of 0.5 ml of a CT nasal pharmaceutical solution (1100 IU) to a glass tube pre-coated with DTPA-dianhydride (0.01 mg) at 25°C with continuous mild stirring for 30 min. Results: After solid phase purification of the radiolabeled hormone, instant thin layer chromatography (ITLC) showed radiochemical purity of higher than 95 % at optimized conditions (specific activity =67-134 KBq/IU, labeling efficiency 70%). 67Ga-DTPA-CT mainly accumulates in the liver. Conclusion: Preliminary in vivo studies (ID/g%) in male wild-type rats showed significant liver uptake of the tracer after 24 hours. 67Ga-DTPA-CT can be a suitable probe for biodistribution study of CT receptors in various physiological as well as neoplastic lesions with over-expressed calcitonin receptors.
Jalilian, A. R., Hosseini, M. A., & Bolourinovin, F. (2011). Preparation and biodistribution study of 67Ga-gallium calcitonin. Iranian Journal of Nuclear Medicine, 19(2), 38-45.
MLA
Amir Reza Jalilian; Mohammad Amin Hosseini; Fatemeh Bolourinovin. "Preparation and biodistribution study of 67Ga-gallium calcitonin". Iranian Journal of Nuclear Medicine, 19, 2, 2011, 38-45.
HARVARD
Jalilian, A. R., Hosseini, M. A., Bolourinovin, F. (2011). 'Preparation and biodistribution study of 67Ga-gallium calcitonin', Iranian Journal of Nuclear Medicine, 19(2), pp. 38-45.
VANCOUVER
Jalilian, A. R., Hosseini, M. A., Bolourinovin, F. Preparation and biodistribution study of 67Ga-gallium calcitonin. Iranian Journal of Nuclear Medicine, 2011; 19(2): 38-45.
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