Impact of reconstruction method on quantitative parameters of 99mTc-TRODAT-1 SPECT

Document Type : Original Article


1 Nuclear Medicine and Molecular Imaging Research Center, Namazi Teaching Hospital, Shiraz University of Medical Sciences, Shiraz, Iran

2 Department of Neurosurgery, Shiraz University of Medical Sciences, Shiraz, Iran


Introduction: Quantitative evaluation is recommended to improve diagnostic ability and serial assessment of dopamine transporter (DAT) density scans. We decided to compare the ordered subsets expectation-maximization (OSEM) with filtered back-projection (FBP), and to investigate the impact of different iteration and cut-off frequencies on SBR values.
Methods: We retrospectively examined 27 consecutive patients. SPECT reconstruction was performed using OSEM and FBP with Chang’s attenuation correction (AC). Iterative reconstruction parameters were used with different iterations ranging from 2, 4, 6, 8, and 10 with fixed 10 subsets and different subsets including 5, 10 and 15 with fixed 6 iterations. Reconstruction with FBP were performed with different critical cut-off frequencies of 0.3, 0.4 and 0.5.
Results: Comparing SBR derived by OSEM reconstruction with 10 subsets but different iterations revealed statistically significant intraclass correlation (ICC) in both right and left side. There is also no significant difference between different OSEM reconstruction with different subsets and ICC was excellent in all patients. ICC for FBP reconstruction with different cut-off frequency revealed good ICC in all patients. However, lower degree of SBR showed higher decrease in ICC with insignificant and poor correlation in patients with SBR Conclusion: Our study showed that change in FBP reconstruction parameters can greatly impact the SBR value of 99mTc-TRODAT-1, especially in patients with more severe disease. However, OSEM reconstruction revealed better reproducibility for SBR using different iterations.


Main Subjects

  1. Papathanasiou N, Rondogianni P, Chroni P, Themistocleous M, Boviatsis E, Pedeli X, Sakas D, Datseris I. Interobserver variability, and visual and quantitative parameters of (123)I-FP-CIT SPECT (DaTSCAN) studies. Ann Nucl Med. 2012 Apr;26(3):234-40.
  2. Darcourt J, Booij J, Tatsch K, Varrone A, Vander Borght T, Kapucu OL, Någren K, Nobili F, Walker Z, Van Laere K. EANM procedure guidelines for brain neurotransmission SPECT using (123)I-labelled dopamine transporter ligands, version 2. Eur J Nucl Med Mol Imaging. 2010 Feb;37(2):443-50.
  3. Badiavas K, Molyvda E, Iakovou I, Tsolaki M, Psarrakos K, Karatzas N. SPECT imaging evaluation in movement disorders: far beyond visual assessment. Eur J Nucl Med Mol Imaging. 2011 Apr;38(4):764-73.
  4. Tossici-Bolt L, Dickson JC, Sera T, Booij J, Asenbaun-Nan S, Bagnara MC, Borght TV, Jonsson C, de Nijs R, Hesse S, Koulibaly PM, Akdemir UO, Koole M, Tatsch K, Varrone A. [123I]FP-CIT ENC-DAT normal database: the impact of the reconstruction and quantification methods. EJNMMI Phys. 2017 Dec;4(1):8.
  5. Dickson JC, Tossici-Bolt L, Sera T, Booij J, Ziebell M, Morbelli S, Assenbaum-Nan S, Vander Borght T, Pagani M,  Kapucu O, Hesse S, Van Laere K, Darcourt J, Varrone A, Tatsch K. The impact of reconstruction and scanner characterisation on the diagnostic capability of a normal database for [(123)I]FP-CIT SPECT imaging. EJNMMI Res. 2017;4:8.
  6. Matsutomo N, Nagaki A, Yamao F, Sasaki M. Optimization of iterative reconstruction parameters with 3-dimensional resolution recovery, scatter and attenuation correction in (1)(2)(3)I-FP-CIT SPECT. Ann Nucl Med. 2015 Aug;29(7):636-42.
  7. Huang WS, Lee MS, Lin JC, Chen CY, Yang YW, Lin SZ, Wey SP. Usefulness of brain 99mTc-TRODAT-1 SPET for the evaluation of Parkinson's disease. Eur J Nucl Med Mol Imaging. 2004 Feb;31(2):155-61.
  8. Koh J, Yamamoto K, Sakata M, Murata K, Ito H. Quantitative reference value of dopamine transporter single-photon emission computed tomography in healthy Japanese older adults. Neurol Clin Neurosci. 2016;4(6):215-219.
  9. Varrone A, Dickson JC, Tossici-Bolt L, Sera T, Asenbaum S, Booij J, Kapucu OL, Kluge A, Knudsen GM, Koulibaly PM, Nobili F, Pagani M, Sabri O, Vander Borght T, Van Laere K, Tatsch K.  European multicentre database of healthy controls for [123I]FP-CIT SPECT (ENC-DAT): age-related effects, gender differences and evaluation of different methods of analysis. Eur J Nucl Med Mol Imaging. 2013;40(2):213-27.
  10. Lin CC, Fan YM, Lin GY, Yang FC, Cheng CA, Lu KC, Lin JC, Lee JT. 99mTc-TRODAT-1 SPECT as a Potential Neuroimaging Biomarker in Patients With Restless Legs Syndrome. Clin Nucl Med. 2016 Jan;41(1):e14-7.
  11. Fallahi B, Esmaeili A, Beiki D, Oveisgharan S, Noorollahi-Moghaddam H, Erfani M, Tafakhori A, Rohani M, Fard-Esfahani A, Emami-Ardekani A, Geramifar P, Eftekhari M. Evaluation of 99m Tc-TRODAT-1 SPECT in the diagnosis of Parkinson’s disease versus other progressive movement disorders. Ann Nucl Med. 2016 Feb;30(2):153-62.
  12. Dickson JC, Tossici-Bolt L, Sera T, Erlandsson K, Varrone A, Tatsch K, Hutton BF. The impact of reconstruction method on the quantification of DaTSCAN images. Eur J Nucl Med Mol Imaging. 2010 Jan;37(1):23-35.
  13. Maebatake A, Imamura A, Kodera Y, Yamashita Y, Himuro K, Baba S, Miwa K, Sasaki M. Evaluation of iterative reconstruction method and attenuation correction in brain dopamine transporter spect using an anthropomorphic striatal phantom. Asia Ocean J Nucl Med Biol. 2016 Summer;4(2):72-80.
  14. Winz OH, Hellwig S, Mix M, Weber WA, Mottaghy FM, Schäfer WM, Meyer PT. Image quality and data quantification in dopamine transporter SPECT: advantage of 3-dimensional OSEM reconstruction? Clin Nucl Med. 2012 Sep;37(9):866-71.
  15. Koch W, Hamann C, Welsch J, Pöpperl G, Radau PE, Tatsch K. Is iterative reconstruction an alternative to filtered backprojection in routine processing of dopamine transporter SPECT studies? J Nucl Med. 2005 Nov;46(11):1804-11.
  16. Van Laere K, De Ceuninck L, Dom R, Van den Eynden J, Vanbilloen H, Cleynhens J, Dupont P, Bormans G, Verbruggen A, Mortelmans L. Dopamine transporter SPECT using fast kinetic ligands: 123I-FP-beta-CIT versus 99mTc-TRODAT-1. Eur J Nucl Med Mol Imaging. 2004 Aug;31(8):1119-27.
  17. Bor-Seng-Shu E, Felicio AC, Braga-Neto P, Batista IR, Paiva WS, de Andrade DC, Teixeira MJ, de Andrade LA, Barsottini OG, Shih MC, Bressan RA, Ferraz HB. Dopamine transporter imaging using 99mTc-TRODAT-1 SPECT in Parkinson's disease. Med Sci Monit. 2014 Aug 11;20:1413-8.