Document Type : Original Article
Department of Nuclear Medicine, Sultan 2 Abdülhamid Han Training and Research Hospital, Istanbul, Turkey
Department of Nuclear Medicine, Faculty of Medicine, Lokman Hekim University, Ankara, Turkey
Department of Physiology, Faculty of Medicine, Tokat Gaziosmanpasa University, Tokat, Turkey
Introduction: The purpose of this study was to evaluate possible preventive role of quercetin on doxorubicin (DOX) induced kidney toxicity using Tc-99m Dimercaptosuccinic Acid ([99mTc]Tc-DMSA) renal cortical scintigraphy and biochemical approaches.
Methods: 28 Male Wistar rats were separated into four groups. First group was intraperitoneally (i.p.) injected saline and regarded as the control group; second one was received 18 mg/kg/i.p doxorubicin for three days at a 24 h interval; the third and last group received 10 mg/kg and 100 mg/kg quercetin for 21 days and for the last 3 days doxorubicin and quercetin were administrated together at the same time. On the 22 th day of the experiment, [99mTc]Tc-DMSA renal cortikal scintigraphy and biochemical parameters were measured.
Results:DOX administration significantly increased blood urea nitrogen (845%) and creatinine (702%) levels in serum; nitric oxide (158%), plasma tumor necrosis factor-alpha (233%) and interleukin-6 (191%) levels in kidney tissue, and also reduced [99mTc]Tc-DMSA uptake by 29% in the kidneys as well. Pre-treatment with quercetin mitigated such alterations in all mentioned parameters.
Conclusion: All data indicate that oxidative stress and inflammatory processes are involved in DOX-induced nephrotoxicity, which might be decreased by quercetin.In addition, [99mTc]Tc-DMSA scintigraphic may be a good method for demonstrating doxorobucin-induced renal injury.