Document Type: Original Article
Radiopharmaceutical Research and Development Laboratory, Nuclear Science and
Technology Research Institute, Atomic Energy organization of Iran, Tehran, Iran
Research Institute for Nuclear Medicine, Tehran University of Medical Sciences, Tehran, Iran
Introduction: In this research, [166Ho]Holmium chitosan complex production is described in details, followed by determination of complex radiochemical purity, stability and biodistribution (after intra-articular injection) in wild-type male rats. Finally a Ho-166 based chitosan kit for ultimate radiosynovectomy as well as radiotherapy applications was developed. Methods: 166Ho-chitosan complex was prepared using chitosan concentrations and 166HoCl3 followed by intra-articular injection and biodistribution studies in wild-type rats including and excluding injected knee. Results: The [166Ho]Holmium chitosan complex was prepared with high radiochemical yield (>95 %) in the optimized condition (35mg/3ml of chitosan in %1 AcOH, pH. 3, >98%, ITLC) was injected to wild-type rats followed by the biodistribution studies of the compound among the tissues excluding the injected knee data. Intra-articular injection of [166Ho]holmium chitosan complex to male wild-type rats and investigation of leakage of activity in the body showed that most of injected dose has remained in injection site 144 h after injection. Conclusion: Successful development and formulation of 166Ho-chitosan kit is described. This kit has the potential for use in clinical setting namely for radiosynovectomy and cancer radiochemotherapy.